• Optimization of Critical Reagents labeling is important for proper Anti-Drug Antibodies (ADA) assessment;
  • Biotin and Sulfo-tag conjugated trastuzumab have discrete optimal molar incorporation ratios;
  • Free unlabeled biotin affects assay performance while free sulfo-tag does not
  • Freeze / thaw cycles negatively impact the Sulfo-tagged trastuzumab performance.


Click Here to read the full Case Study by Agostinho G. Rocha, PhD*; Anthony Mancino; Matthew Sciscione; Katelyn Krynski; Jason Vergara; Christopher Beaver, PhD Syneos Health, Princeton, NJ, USA

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